Cryopreservation of Isolated Mouse Germinal Vesicles
نویسندگان
چکیده
منابع مشابه
The Effect of FBS Concentration on Cryopreservation of Isolated Spermatogonial Cells from Neonatal Mouse by Macs Method
Purpose: The purpose of this study was the separation of spermatogonial cells from prepubertal NMRI mouse testis by magnetic activating cell sorting (MACS) method and assessment of FBS concentration on surviving these cells after cryopreservation. Materials and Methods: Testes from 6-days-old mouse removed and digested in two enzymatic mediums. First 8-9 min at medium containing collagenase, tr...
متن کاملAXENIC CULTURE AND CRYOPRESERVATION OF GIARDIA LAMBLIA ISOLATED IN IRAN
Giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. The cysts were harvested and purified using the sucrose gradient method. IS The excystation of G. lamblia was obtained by treating them with HCI (0.01N)', then they were transferred on to modified TYI-S-33 medium. Out of 55 stool samples 17 cases were excysted with a rate of ...
متن کاملIsolated Chromaffin Vesicles
Isolated secretory vesicles from bovine adrenal medulla contain 80 nmol of Ca2+ and 25 nmol of Mg2+ per milligram of protein. As determined with a Ca2+-selective electrode, a further accumulation of about 160 nmol of Ca2+/mg of protein can be attained upon addition of the Ca2+ ionophore A23187. During this process protons are released from the vesicles, in exchange for Ca2+ ions, as indicated b...
متن کاملP-29: Effects of Cumulus Cells on Survival, In vitro Maturation,Fertilization and Subsequent Developmental Capacity of Mouse Germinal Vesicle Stage Oocytes during Vitrification
Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...
متن کاملGerminal vesicle breakdown in the mouse oocyte.
Germinal vesicle breakdown in mouse oocytes in vivo and in vitro has been examined by electron microscopy. In vitro oocytes were studied immediately after release from follicles and at various times (05-11 h) in culture. Approximately 30 min after oocyte release, chromatin condensation begins along the convoluted nuclear envelope (NE). Chromatin granules are common in all condensing chromosomes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2005
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.16071